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rice pollen sterility calculation method
Term Information
Accession
CO_320:0000326
Name
rice pollen sterility calculation method
Ontology
CO_320_rice
Synonyms
None
Alternate IDs
None
Definition
Collect pollen from at least ten florets at anthesis, fix in 70% alcohol. Place 2-3 anthers from each of five florets on a glass slide, and squeeze the pollen out in a drop of 1% iodine potassium iodide (I2-KI) solution with a spear-shaped needle. Fertile pollen grains will be round and well stained, sterile pollen grains will be unstained or partially stained, and withered or round. Using a microscope at magnification 10x10, count fertile and sterile grains in at least three microscopic fields, calculate the proportion that are sterile
[source: unknown]
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CO_320:0000326 rice pollen sterility calculation method